Identifying kinetically stable proteins with capillary electrophoresis

Kinetically Stable Proteins

Capillary Electrophoresis of PEGylated Proteins

Capillary electrophoresis has become a well-established technique for evaluating the composition and concentrations of a wide range of protein samples. In addition, capillary electrophoresis can be used to study a variety of protein properties including the net protein charge, the extent of ion binding, and the presence of specific biomolecular interactions. This wide range of applications is p...

Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE.

Most proteins are in equilibrium with partially and globally unfolded conformations. In contrast, kinetically stable proteins (KSPs) are trapped by an energy barrier in a specific state, unable to transiently sample other conformations. Among many potential roles, it appears that kinetic stability (KS) is a feature used by nature to allow proteins to maintain activity under harsh conditions and...

Capillary electrophoresis of proteins and peptides.

Capillary electrophoresis (CE) is a high-resolution technique for the separation of a wide variety of molecules of biological interest such as metabolites, drugs, amino acids, nucleic acids, and carbohydrates. This unit focuses on the use of CE to separate proteins and peptides based on charge-to-mass ratios. Separation of proteins based on their isoelectric points is described along with a pro...

High-performance capillary electrophoresis of cereal proteins.

Cereal grains are widely used of human foods and animal feed throughout the world. Cereals provide dietary protein, which also often has a functional role, as wheat gluten does in bread. Cereal proteins are unique in many ways: they are highly complex and heterogeneous, are often difficult to extract, and aggregate readily, making them difficult to characterize. Because of the economic importan...